Restriction digests of the clone give the following sizes (kb): SacI/XbaI--6.3, 0.45; EcoRI--4.3, 2.3; KpnI--5.1, 1.6. The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose and is induced at a moderate level by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
