五月婷婷激情四射,激情五月婷婷小说,国产欧美日韩在线

▍最近瀏覽歷史

[清空]

▍更多產(chǎn)品

貨號：B245025
名稱：Acanthamoeba healyi Moura et al.

貨號：B245024
名稱：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號：B245023
名稱：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號：B245022
名稱：Eutypella vitis (Schweinitz) Cooke emend. Glawe

貨號：B245021
名稱：Streptomyces scabiei (Thaxter) Lambert and Loria

貨號：B245020
名稱：Graphium cuneiferum (Berkeley et Broome) Mason et Ellis

貨號：B245019
名稱：HFBCI28

貨號：B245018
名稱：Septoria passiflorae Sydow, anamorph

貨號：B245017
名稱：Dictyostelium purpureum Olive

貨號：B245016
名稱：Y71

▍聯(lián)系我們

0574-87157013
mingzhoubio@163.com
浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
創(chuàng)e慧谷42號樓B幢401室

Giardia intestinalis (Lambl) Alexeieff

規(guī)格:

貨期:

編號:B216066

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產(chǎn)品名稱	Giardia intestinalis (Lambl) Alexeieff
商品貨號	B216066
Strain Designations	WB clone A6
Application	Enteric Research Food and waterborne pathogen research
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	Unknown
Product Format	frozen
Storage Conditions	Frozen Cultures: -70°C for 1 week; liquid N₂ vapor for long term storage Freeze-dried Cultures: 2-8°C Live Cultures: See Protocols section for handling information
Type Strain	no
Comments	Antigenic variation in vivo Antigenic variation Association of variant surface antigens with thick cell coat Induction of differentiation by cholesterol starvation Variant specific epitopes
Medium	ATCC^® Medium 2695: Keister's Modified TYI-S-33 ATCC^® Medium 2155: LYI Giardia Medium (filtered)
Growth Conditions	Temperature: 35°C Culture System: Axenic
Cryopreservation	Harvest and Preservation Harvest cells from a culture that is at or near peak density. To detach cells from the wall of the culture tubes place on ice for 10 minutes. Invert tubes several times until the majority of the cells are in suspension. Centrifuge tubes at 800 x g for 5 minutes. Adjust the concentration of cells to 2 x 10⁷/mL in fresh medium. Before centrifuging prepare a 24% (v/v) solution of sterile DMSO in fresh medium containing 8% (w/v) sucrose. The solution is prepared as follows: Add 1.05 g sucrose to 10 mL of fresh medium and filter sterilize through a 0.2 µm filter; Add 2.4 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube; Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 7.6 mL of ice cold medium prepared in step 3a. The final concentration will be 24% (v/v) DMSO and 8% (w/v) sucrose; Invert several times to dissolve the DMSO; Allow to warm to room temperature. Mix the cell preparation and the cryoprotective agent, prepared in step 3, in equal portions. Thus, the final concentration will equal 12% (v/v) DMSO + 4% sucrose (w/v) and 10⁷ cells/mL. The time from the mixing of the cell preparation and DMSO stock solution to the start of the freezing process should be no less than 15 min and no longer than 30 min. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.) The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing 13 mL ATCC Medium 2695. Incubate the culture on a 15º horizontal slant at 35°C.
Name of Depositor	TE Nash
Special Collection	NCRR Contract
References	Aggarwal A, Nash TE. Antigenic variation of Giardia lamblia in vivo. Infect. Immun. 56: 1420-1423, 1988. PubMed: 3372015 Nash TE, et al. Antigenic variation in Giardia lamblia. J. Immunol. 141: 636-641, 1988. PubMed: 2454999 Pimenta PF, et al. Variant surface antigens of Giardia lamblia are associated with the presence of a thick cell coat: Thin section and label fracture immunocytochemistry survey. Infect. Immun. 59: 3989-3996, 1991. PubMed: 1937758 Lujan HD, et al. Cholesterol starvation induces differentiation of the intestinal parasite Giardia lamblia. Proc. Natl. Acad. Sci. USA 93: 7628-7633, 1996. PubMed: 8755526 Nash TE, et al. Variant specific epitopes of Giardia lamblia. Mol. Biochem. Parasitol. 42: 125-132, 1990. PubMed: 1700296 Miller RL, et al. Identification of Giardia lamblia Isolates Susceptible and Resistant to Infection by the Double-Stranded RNA Virus. Exp. Parasitol. 66: 118-123, 1988. PubMed: 3366209

序號	文章標(biāo)題	操作
1	ATCC細(xì)胞運(yùn)輸和處理	查看詳情
2	其他海外品牌代理	查看詳情
3	ATCC代理	查看詳情
4	購買須知	查看詳情
5	訂購流程	查看詳情

梅經(jīng)理	17280875617	1438578920
胡經(jīng)理	13345964880	2438244627
周經(jīng)理	17757487661	1296385441
于經(jīng)理	18067160830	2088210172
沈經(jīng)理	19548299266	2662369050
李經(jīng)理	13626845108	972239479

<center id="ooers"></center>