Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 1.05; HindIII--3.9; PstI--2.6, 1.3; ClaI--3.9; XhoI--2.8, 1.1. Exact restriction sizes are as follows: BamHI--2.98 kb, 1.03 kb; HindIII--4.01 kb; PstI--2.59 kb, 1.295 kb; ClaI--4.01 kb; XhoI--2.92 kb; 1.09 kb. Contains the complete coding sequence and a poly(A) tract of 0.1 kb. The insert contains PstI, BglI, and ApaI sites in the coding region and NcoI and AhaIII sites in the non-coding region. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
