-20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Introduction
Cell Line Description:
SNU-182 was derived in 1989 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy.
Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.
See www.atcc.org for more information
Quality Control Specifications
> 100 kb in size by agarose gel electrophoresis
No RNA detected in the agarose gel
A260/A280 ratio ≥ 1.8
Tested and verified for PCR amplification and restriction digestion.
