Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 1.2, 0.9; BamHI--4.8; PstI--2.9, 1.9. Restriction digests of the clone give the following sizes (kb): BglII--4.3; EcoRI--2.9, 1.3; PstI--3.8, 0.5; PvuII--2.5, 1.1, 0.4 (doublet); SacI--3.1, 1.2. The insert contains the following restriction sites (bp from the 5' end): BglII--310; PstI--852; PvuII--97, 436; SacI--168. Contains the complete coding sequence, including 122 nt of 5' untranslated region (with at least one translational stop codon in each reading frame), and 204 nt of 3' untranslated region. The GenBank accession number for the genomic sequence is M99703. This record contains correct information for 4 sites in the cDNA sequence (2 in coding regions, 2 in non-coding regions) that were reported in error. Transcription from the T3 promoter gives the sense strand. |
