Plasmid can replicate in most gram-negative bacteria except Bacteroides.
Constructed to provide tight but inducible regulation from the trp promoter on a high copy number plasmid.
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References
Warne SR, et al. Use of a modified Escherichia coli trpR gene to obtain tight regulation of high-copy-number expression vectors. Gene 46: 103-112, 1986. PubMed: 3026921
