| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--24.0, 20.0, 0.35, 0.28 (doublet); BamHI--16.0, 11.0, 6.5 (doublet), 5.6; BglII--19.0, 15.0, 7.0, 2.4, 0.7; EcoRV--5.6, 5.3, 4.5 (doublet), 3.7 (doublet), 3.3, 3.1, 2.5, 1.5, 1.4 (doublet), 1.3, 0.75, 0.6 (doublet); HindIII--21.0, 9.4, 6.0, 4.4, 3.8, 0.6; NdeI--20.0, 5.8 (doublet), 3.8, 2.4, 2.2, 1.7, 1.6, 0.55. The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.61; EcoRI--0.27, 0.50, 1.57; EcoRV--0.53, 1.51; HincII--1.12; HindIII--0.13; NdeI--0.87; SphI--1.76. Corresponds to nt 804-2548 of the GenBank record. It does not include the complete coding sequence (nt 759-1271). Detects a 2.8-2.9 kb mRNA in rabbit brain, and a slightly smaller transcript in murine muscle and kidney. |
