| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--9.0, 7.0, 3.0, 2.7 (triplet), 2.4 (doublet), 2.1, 1.4, 1.2 (doublet), 1.1, 0.9, 0.8 and smaller bands. Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.820 (vector), 3.015, 2.820, 2.721, 2.704, 2.572, 2.479, 2.142, 1.362, 1.244, 1.210, 1.106, 0.962, 0.841, 0.662, 0.639, 0.548, 0.501 (vector), 0.450, 0.435. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 45593-82896 of the sequence of chromosome VIII. |
