| Vector |
Construct size (kb): 4.949999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D6S1248E
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: 6
Tissue: BL29 cell line
Disease: Burkitt's lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 2.05
Cross references: DNA Seq. Acc.: T25352; EST: EST041; Primer: T7
DNA Seq. Acc.: T25353; EST: EST041R; Primer: T3 Insert lengths(kb): 2.049999952316284 Tissue: BL29 cell line Burkitt's lymphoma Disease: Burkitt's lymphoma Gene product: DNA Segment, single copy, expressed [D6S1248E] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.35, 0.8; BamHI--3.2, 1.75; HindIII--4.1, 0.85; KpnI--5.0; PstI--3.1, 1.6, 0.3. |
