| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--10.3, 6.8, 5.0 (triplet), 2.9, 2.7, 1.8, 1.5, 1.4, 1.2 and smaller bands. The insert reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.437, 6.033, 5.139, 4.893, 4.870, 3.685, 3.431, 2.924, 2.742, 2.493, 2.474, 1.842, 1.761, 1.565, 1.408, 1.311, 1.214, 1.170, 1.145, 0.947, 0.835, 0.827, 0.811, 0.800, 0.793, 0.583, 0.412, 0.376, 0.325, 0.241, 0.180, 0.084. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
