| 產品名稱 |
pVL-RabGGTbeta |
| 商品貨號 |
B219165 |
| Designations |
pVL-RabGGTbeta |
| GenBank Number |
L10416
|
| Species |
Rattus norvegicus, rat |
| Depositors |
MC Seabra, S Armstrong |
| Applications |
in suitable host, produces protein rab geranylgeranyl transferase beta Rab geranylgeranyl transferase, beta subunit |
| Vector |
Construct size (kb): 11.0 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.299999952316284 Tissue: brain Gene product: rab geranylgeranyl transferase beta(Rab geranylgeranyl transferase, beta subunit) [GNGNT2B*] Target Gene: rab geranylgeranyl transferase beta |
| Insert Size (kb) |
1.300 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; BamHI--9.8, 0.9; XhoI--7.7, 2.3. The insert contains the following restriction sites (approximate kb from the 5' end): XhoI--0.07; BglII--0.59,1.14; BamHI--0.87; BclI--1.29. When pVL-RabGGTalpha and pVL-RabGGTbeta (ATCC 87154, 87155) are used to generate recombinant baculoviruses and infect Sf9 cells, the active dimer is produced. |
| References |
Cremers FP, et al. REP-2, a Rab escort protein encoded by the choroideremia-like gene. J. Biol. Chem. 269: 2111-2117, 1994. PubMed: 8294464
Luckow VA, Summers MD. Signals Important for high-level expression of foreign genes in Autographa californica Nuclear Polyhedrosis Virus expression vectors. Virology 167: 56-71, 1988. PubMed: 3142147
|
