| Comments |
Insert contains internal PvuII (1), PstI (2), BglII (2) and BamHI (1) sites. Restriction digests of the clone give the following sizes (kb): SphI--4.4, 2.2; EcoRI--6.6; HindIII--6.6; BamHI--6.0, 1.0; PstI--3.6, 1.65, 1.2. Clone of the 5'-end of HMG-CoA including the 5' initiation sites and exon 1. Enzyme(s) not detecting polymorphism: ApaI, ApaLI, Asp718, AvaII, BamHI, BglI, EcoRI, NcoI, NheI, PstI, PvuII, SacI, SpeI, StuI (8 unrelated individuals). |
| References |
Luskey KL. Conservation of promoter sequence but not complex intron splicing pattern in human and hamster genes for 3-hydroxy-3-methylglutaryl coenzyme A reductase. Mol. Cell. Biol. 7: 1881-1893, 1987. PubMed: 3037337
Leitersdorf E, Luskey KL. HgiAl polymorphism near the HMGCR promoter. Nucleic Acids Res. 18: 5584, 1990. PubMed: 1977131
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