| 產品名稱 |
pG57/T1 |
| 商品貨號 |
B219559 |
| Designations |
pG57/T1 |
| GenBank Number |
M61773
|
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
A Tzagoloff |
| Applications |
in another host, produces protein mitochondrial protein, required for assembly of F1 ATPase |
| Vector |
Construct size (kb): 16.79998970031738 |
| Insert |
DNA: genomic Insert lengths(kb): 9.0 Gene product: mitochondrial protein, required for assembly of F1 ATPase [ATP12] Target Gene: mitochondrial protein, required for assembly of F1 ATPase |
| Insert Size (kb) |
9.0 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): SmaI/SalI--10.0, 8.0; EcoRI--6.6, 3.6 (doublet), 2.3, 0.9; PstI--13.5, 2.9. The insert contains the following restriction sites (approximate kb from the end closer to the vector SmaI site [nt of the published sequence]): XbaI--1.1, 2.8; EcoRI--1.6, 5.3, 6.3 [193]; SstI--6.1 [2]; HindIII--7.5 [1425]. The EcoRI site at nt 193 is within the reading frame. |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References |
Bowman S, et al. Characterization of ATP12, a yeast nuclear gene required for the assembly of the mitochondrial F1-ATPase. J. Biol. Chem. 266: 7517-7523, 1991. PubMed: 1826907
|
