| 產品名稱 |
pAGRBglR2 [NEB731] |
| 商品貨號 |
B220459 |
| Designations |
pAGRBglR2 [NEB731] |
| GenBank Number |
I13180
|
| Species |
Bacillus globigii Migula |
| Depositors |
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications |
produces protein modification methylase BglII produces protein restriction endonuclease BglII |
| Vector |
Construct size (kb): 8.5 |
| Insert |
DNA: genomic Insert lengths(kb): 1.799999952316284 Gene product: restriction endonuclease BglII [hsdM] Target Gene: modification methylase BglII, restriction endonuclease BglII |
| Insert Size (kb) |
1.800 |
| Media |
ATCC Medium 1065 (see below) plus ampicillin (100.0 ug/ml) plus kanamycin (50.0 ug/ml) plus tetracycline (10.0 ug/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of pSYXBglM (weaker) give the following sizes (kb): EcoRI--uncut; BamHI--7.2, 1.6; HindIII--4.8, 3.7; PstI--8.2. Restriction digests of pAGRBglR2 give the following sizes (kb): EcoRI--uncut; BamHI--7.2; HindIII--7.4; PstI--7.8. This contains two plasmids, one encoding the methylase and one encoding the restriction endonuclease. To optimize growth and expression, grow in LB containing carbenicillin, kanamycin, and tetracycline at 30C until late log phase. Induce with IPTG and grow 20 more hrs at 30C until harvest. |
| References |
Brooks JE, et al. Method for cloning and producing the BglII restriction endonuclease and modification methylase. US Patent 5,434,068 dated Jul 18 1995
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
