Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
MATa can1-100 ade2-1 his3-11 his3-15 leu2-3 leu2-12 trp1-1 ura3-1 deltahsp104::LEU2+
Preceptrol®
no
Mating Type
a
Ploidy
haploid
Comments
phenotype: CanS Ade- His- Leu+ Trp- Ura- Hsp104-
ApaI-BglII fragment of hsp104 gene replaced by LEU2 gene then integrated into W303 to replace wild-type hsp104
Induces heat-shock protein hsp101 for tolerance to heat, ethanol, and other stresses
Medium
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 25.0°C
Name of Depositor
SL Lindquist
Special Collection
NCRR Contract
Chain of Custody
ATCC <<--SL Lindquist<<--Y. Sanchez
References
Parsell DA, et al. Hsp104 is highly conserved protein with two essential nucleotide-binding sites. Nature 353: 270-273, 1991. PubMed: 1896074
Sanchez Y, Lindquist SL. HSP104 required for induced thermotolerance. Science 248: 1112-1115, 1990. PubMed: 2188365
