| Insert |
DNA: cDNA Insert lengths(kb): 0.7609999775886536 Tissue: MethA fibrosarcoma cell line Gene product: lymphocyte antigen-6 [DAG] Alleles: 6.1, 6.1, 6.1, 6.2, 6.2, 6.2, 6.1, 6.1, 6.2, 6.2, 6.1, 6.2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--2.3, 1.15; EcoRI--2.7, 0.8; PstI--3.2, 0.35; PvuII--2.4, 1.15; SacI--2.9, 0.3 (doublet). The insert contains the following restriction sites (nt from the 5' end): SacI--160, 467; PstI--480. Encodes Ly-6E.1 (Ly-6a allele) recognized by the SK70.94 and HD42 monoclonal antibodies. Contains the complete coding sequence (nt 89-492) and extends from a linker-derived EcoRI site at the 5' end to a naturally occurring EcoRI site in the 3' untranslated region. |
