| 產品名稱 |
pMAM20 |
| 商品貨號 |
B220924 |
| Designations |
pMAM20 |
| Species |
Strongylocentrotus purpuratus, purple sea urchin |
| Depositors |
B Polisky |
| Applications |
produces protein ectoderm protein (spec) |
| Vector |
Construct size (kb): 3.400000095367432 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.3899999856948853 Tissue: embryo Gene product: ectoderm protein (spec) Target Gene: ectoderm protein (spec) |
| Insert Size (kb) |
0.390 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
This clone encodes 5/6 of the spec1c gene. The depositor indicates it's the specc6(1b) gene. The rop-spec fusion polypeptide is highly expressed at 42 C and can be used as a control for amplification of rop fusion polypeptides. |
| References |
Muesing M, et al. High-level expression in Escherichia coli of calcium-binding domains of an embryonic sea urchin protein. Gene 31: 155-164, 1984. PubMed: 6098520
|
