Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes. The library covers the region 2p11.1-2q11.1. Approximately 50% of the clones contain repetitive sequences. Of 46 single copy clones tested, 37 (80%) hybridized to chromosome 2. Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19. |
