| 產品名稱 |
pLD55 [FD 27748] |
| 商品貨號 |
B221423 |
| Designations |
pLD55 [FD 27748] |
| Depositors |
Pfizer, Inc. |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 0E-16 Vector: pLD55 (plasmid) Construction: pBR322, Y. lipolytica NRRL Y-1094 Marker(s):LEU2,URA3,ampR Construct size (kb): 0.0 Features: marker(s): ampR, LEU2, URA3
replicon: pMB1 |
| Applications |
YI-type (integrating) shuttle vector contains sequence 3-isopropylmalate dehydrogenase beta-isopropylmalate dehydrogenase contains sequence uridine monophosphate synthetase UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1 integrating vector shuttle vector |
| Comments |
Contains plasmid pLD40 is pBR322 containing a 2.3 kb EcoRI fragment (partial) with the LEU2 gene inserted at the EcoRI site. Constructed by inserting a Sau3AI (partial) fragment into the BamHI site of pLD40. |
| Media |
ATCC® Medium 1245: YEPD
|
| Growth Conditions |
Temperature: 25.0°C |
| References |
Davidow LS, DeZeeuw JR. Process for transformation of Yarrowia lipolytica. US Patent 4,880,741 dated Nov 14 1989
|
| Shipped |
freeze-dried |
