| 產品名稱 |
pCLL34 |
| 商品貨號 |
B221474 |
| Designations |
pCLL34 |
| Depositors |
American Cyanamid Co., JS Feitelson, American Cyanamid Co. |
| Isolation |
Constructed by inserting a 844 bp BclI fragment from pIJ486 containing the fd terminator and a polylinker into the BamHI site of pARC1. |
| Applications |
Promoter-cloning vector Vector permitting visual detection of activity in colonies Promoter-cloning vector permitting visual detection of activity by expression of a brown pigment. |
| Vector |
Size (kb): 19.7999897003173800 Vector: pCLL34 (plasmid) Construction: pARC1, pIJ486 Marker(s):tsrR Construct size (kb): 19.79998970031738 Features: insert detection: brown
marker(s): tsrR
terminator: fd |
| Media |
ATCC® Medium 1339: R2 YE medium
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--16.71, 3.41. The additional 35 bp of MCS between the BamHI site and the start of the brown genes do not interfere with gene expression. Contains plasmid pCLL34 Constructed by inserting a 844 bp BclI fragment from pIJ486 containing the fd terminator and a polylinker into the BamHI site of pARC1. The order of the major features in this plasmid is: fd terminator - MCS - brown genes - tsrR. |
| References |
Feitelson JS. Promoter-probe plasmid for analysis of transcriptional regulation. US Patent 4,994,389 dated Feb 19 1991
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
