| Insert |
DNA: cDNA Insert lengths(kb): 1.679999947547913 Gene product: DNA fragmentation factor, 45 kDa subunit [DFF45*] Target Gene: DNA fragmentation factor, 45 kDa subunit |
| Comments |
Restriction digests of the clone give the following sizes (kb): Uncut: EcoRI--3.9, 1.7; BamHI--4.6, 1.0; PvuII--2.6, 1.4, 0.9, 0.4, 0.3. Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.04; BglI--0.00, 1.47; BglII--0.45; KpnI--0.08; NdeI--1.30; NcoI--1.47; PstI--0.57, 0.63, 0.94, 1.01; PvuII--0.44, 0.63; StuI--0.19, 0.92, 0.99; SacI--0.93. |
| References |
Liu X, et al. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 89: 175-184, 1997. PubMed: 9108473
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