| 產品名稱 |
pMW13 [MWPEC13-60] |
| 商品貨號 |
B221564 |
| Designations |
pMW13 [MWPEC13-60] |
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
Miwon Company, Ltd., KS Han, Miwon Company, Ltd. |
| Applications |
produces protein 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase produces protein chorismate mutase-P-prephenate dehydrogenase |
| Insert |
DNA: genomic Gene product: 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase [aroF] Target Gene: chorismate mutase-P-prephenate dehydrogenase, 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--4.2; PstI--4.2; EcoRI/PstI--4.2; HindIII/PstI--2.4, 1.8. Produces phenylalanine optimally in the temperature range of 30C-32C. The order of the major features in the plasmid is: kanR - cI857-10 - lambda PR promoter - lambda PL promoter - pheA - aroF. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Lee SB, et al. Method for production of L-phenylalanine by recombinant E. coli. US Patent 5,008,190 dated Apr 16 1991
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
