Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
MC1061p3 has amber mutations in the genes conferring amp and tet resistance. It is an exconjugant of MC1061 with W3110p3.
Medium
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
Duration: The above host transformed with PcEXV-D2 plasmid should be selected with Amp 50/Tet 15
Name of Depositor
HV Huang
References
. . Nucleic Acids Res. 11: 24-27, 1983.
Lutz CT, et al. Syrinx 2A: An improved lambda phage vector designed for screening DNA libraries by recombination in vivo. Proc. Natl. Acad. Sci. USA 84: 4379-4383, 1987. PubMed: 2955406
