Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 4.3, 1.7; EcoRI--8.2, 3.0, 2.5; SalI--9.4, 4.3. The clone was derived from unintegrated DNA from an acute infection of QT-6 cells. Unintegrated DNA isolated from an acute infection of QT-6 cells by SRA-2 virus. Permuted SRA-2 molecule from lambda gt WES was circul A gag-specific probe can be generated from a 1.35 kb BamHI fragment; a pol-specific probe can be generated from a 0.8 kb HindIII/BamHI fragment; an LTR-specific probe from a 0.32 kb EcoRI fragment, and a src-specific probe from a 0.8 kb PvuII fragment. SRA-2 from lambdagtWES (linearized at SacI) was circularized, cut with SalI, and cloned into pBR322. |
