| 產品名稱 |
Hp2010 |
| 商品貨號 |
B222154 |
| Designations |
Hp2010 |
| GenBank Number |
Y00724
|
| Species |
Homo sapiens, human |
| Depositors |
H Kuivaniemi |
| Vector |
Construct size (kb): 7.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: SphI
Vector end: SphI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: COL1A2
Genomic copy number: unique
Gene name: collagen, type I, alpha 2
Contains complete coding sequence?: Y
Chromosome: 7; Localization: 7 q21.3-q22.1
Tissue: placenta
Type of DNA: cDNA
Insert end: SphI linkers
Insert end: SphI linkers
Insert size (kb): 4.6
Cross references: DNA Seq. Acc.: Y00724
Nucleotides 1-2372 of the insert correspond to
nucleotides 1-2372 of Y00724. Insert lengths(kb): 4.599999904632568 Tissue: placenta Gene product: collagen, type I, alpha 2 [COL1A2] Alleles: A1, B1, K1, C1, A2, C2, K2, b2 |
| Insert Size (kb) |
4.600 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.9; NcoI--5.9, 1.4; PstI--4.4, 2.4, 0.48; SphI--4.6, 2.7; XhoI-- 7.3. Insert contains the following restriction sites (approximate kb from the 5' end): AvaI--1.7, 2.3; EcoRI--4.3; NcoI--1.8, 3.3; PstI--0.45, 3.05; PvuII--0.4, 0.5, 0.8, 1.1, 1.5, 2.4; RsaI--2.7, 3.4; XhoI--2.8. This insert contains a PvuII site in exon 25 (nt 1521) that has been shown to be polymorphic. |
| References |
Constantinou CD, et al. A HaeIII polymorphism at the D10S101 locus. Nucleic Acids Res. 18: 5577, 1990. PubMed: 1977119
Kuivaniemi H, et al. Structure of a full-length cDNA clone for the prepro alpha2(I) chain of human type I procollagen. Biochem. J. 252: 633-640, 1988. PubMed: 3421913
|
