| 產品名稱 |
Human rhinovirus 57 |
| 商品貨號 |
B222181 |
| Classification |
Picornaviridae, Rhinovirus, Human rhinovirus A |
| Agent |
Human rhinovirus 57 |
| Strain |
Ch47 |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
?-70°C or colder |
| Comments |
Virus growth is optimal at pH 6.8-7.3, in roller or rocker cultures incubated at approximately 33°C. All members of the rhinovirus group are ether resistant. |
| Effect on Host |
Cytopathic effects (CPE) in culture include rounding and sloughing of cells ?CPE in culture include cell rounding and sloughing |
| Growth Medium |
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
|
| Recommended Host |
Production Host - H1-HeLa cells (ATCC CRL-1958)
Recommended Host: ?H1 HeLa (ATCC? CRL-1958™) Alternate Host(s) - WI-38 cells (ATCC CCL-75); MRC-5 cells (ATCC CCL-171); HeLa cells (ATCC CCL-2); other human cells |
| Growth Conditions |
Host - H1 HeLa cells (ATCC CRL-1958)
Medium - EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020) Infection conditions - For best results, plate cells 24-48 hours in advance. Infect at about 80% confluence using a multiplicity of infection (MOI) of 0.1 to 1.0. Adsorb virus to cells in a small volume for one hour. After adsorbtion, feed with sufficient medium to maintain cells and incubate for 1 to 7 days at 33 °C, with rocking, in a humidified atmosphere with 5% CO2 . Harvest culture fluid or fluid and cells when entire culture displays cytopathic effects. Preservation - Freeze as quickly as possible and store at -70°C or colder. |
| Name of Depositor |
NIAID, ATCC |
| Special Collection |
ATCC |
| Source |
Derived by passage of NIAID reagent V-112-001-021 (ATCC VR-1167) at ATCC |
| References |
NIAID Catalog of Research Reagents 1978-1980. S. Cunningham, ed. US Department of Health Education and Welfare, Public Health Service, National Institutes of Health; DHEW Pulication No. (NIH) 78-899, Bethesda, MD.
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