Marker(s): ampR, cbxR Promoter: gap Replicon: pMB1, UARS
Applications
Expression vector
Shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 0.2; SacI--5.9; BamHI--5.9.
Available for research purposes only.
One of a series of vectors (ATCC 77463-77465 and 77468-77471) differing only in the selectable marker and the orientation of the gap promoter.
The sequence conferring resistance to carboxin (cbxR) is a mutation of an iron-sulfur protein subunit of the Ustilago maydis succinate dehydrogenase gene.
Shuttle expression vector using the Ustilago maydis gap (glyceraldehyde phosphate dehydrogenase) promoter to drive expression of the cloned insert.
The order of the major features in the plasmid is: Ustilago maydis ARS - ampR - pMB1 ori - 5' lacZ fragment - EcoRI - SacI - gap promoter -> BamHI <- cbxR - 3' lacZ fragment.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References
Kinal H, et al. A family of Ustilago maydis expression vectors: new selectable markers and promoters. Gene 127: 151-152, 1993. PubMed: 8486284
Kinal H, et al. An expression vector for the phytopathogenic fungus, Ustilago maydis. Gene 98: 129-134, 1991. PubMed: 2013404
