| Comments |
The insert includes the following restriction sites (position according to the published sequence): PstI--677, 1859; NcoI--687, 2447; StuI--1111; HindIII--2216. Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 1.8; HindIII--4.5, 0.32; SmaI--4.8; PstI--3.8, 1.2; XhoI--4.8. The insert extends from a site 22 base pairs 5' to the PstI site at nucleotide 677 of the published sequence through the 3' end. It encodes amino acids 220 through 536 and includes the 3' untranslated region. Enzyme(s) not detecting polymorphism: BglI, BglII, DraI, EcoRI, MspI, PstI (20 unrelated X chromosomes), and TaqI (10 unrelated X chromosomes). |
| References |
Beckmann H, et al. TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif. Genes Dev. 4: 167-179, 1990. PubMed: 2338243
Puck JM, et al. A high-frequency RFLP at the human TFE3 locus on the X chromosome. Nucleic Acids Res. 19: 684, 1991. PubMed: 1672758
Paula S Henthorn, personal communication
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