Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7. Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation. pOM3 (ATCC 77222) and pOM4 (ATCC 77223) differ in the orientation of the polylinker. Contains an additional ATG in the inserted ClaI linker in a different reading frame which might also function as start codon for expression. |
