| 產(chǎn)品名稱(chēng) |
pSY2501 |
| 商品貨號(hào) |
B222706 |
| Designations |
pSY2501 |
| GenBank Number |
I02222
|
| Species |
Homo sapiens, human |
| Depositors |
Cetus Corp., A Belt, Cetus Corp. |
| Applications |
produces protein interferon, beta 1, fibroblast |
| Vector |
Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTRP3
Intact vector size: 4.460
Type of vector: plasmid
Vector end: HindIII
Vector end: BamHI
Cloning sites: HindIII
Polylinker sites:
Other unique sites: BamHI
Construction: pVH153, pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: trp, ->
ribosome-binding site: , ->
restriction site: HindIII, ->
marker(s): tetR, ->
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNB1
Other gene symbols: IFNB
Genomic copy number: unique
Gene name: interferon, beta 1, fibroblast
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9 p22
Type of DNA: cDNA
Insert end: HindIII
Insert end: XhoII
Insert size (kb): 0.5
Cross references: DNA Seq. Acc.: I02222 Insert lengths(kb): 0.5 Gene product: interferon, beta 1, fibroblast [IFNB] Target Gene: interferon, beta 1, fibroblast |
| Insert Size (kb) |
0.5 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Contains an Escherichia coli trp promoter regulating expression of complete coding sequence. Mutated codon changes Cys17 to Ser17. Contains 4 internal HinfI and 1 PstI sites. Constructed by ligating HindIII/XhoII fragment into HindIII/BamHI-cut vector (destroying XhoII/BamHI site); HindIII/XhoII digest gives nearly complete insert. |
| References |
Lin LS, et al. Human recombinant interleukin-2-muteins. US Patent 4,518,584 dated May 21 1985
Gelfand DH, et al. Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element. US Patent 4,666,848 dated May 19 1987
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
