| 產(chǎn)品名稱 |
pTL-0059 |
| 商品貨號 |
B222851 |
| Designations |
pTL-0059 |
| Species |
Tobacco etch virus |
| Depositors |
WG Dougherty |
| Vector |
Construct size (kb): 8.989999771118164 |
| Insert |
DNA: cDNA Insert lengths(kb): 5.909999847412109 Gene product: VPg, 6 kDa |
| Insert Size (kb) |
5.910 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--9.0; PstI--9.0; HindIII--5.2, 2.25, 1.1 + others; EcoRI--9.0. The vector (pTL-8) allows in vitro expression of the insert as a fusion protein. The insert includes TEV cDNA sequences from nt 0 to 5915. |
| Classification |
Potyvirus |
| References |
Allison R, et al. The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9-20, 1986.
Carrington JC, et al. Vectors for cell-free expression and mutagenesis of protein-coding sequences. Nucleic Acids Res. 15: 10066, 1987. PubMed: 3697076
|
