| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.511, 1.475, 4.032, 0.888, 0.457, 3.454, 8.355, 2.012, 5.306, 0.882, 0.413, 1.999, 0.976, 1.634, 1.517. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.86, 0.98, 1.5, 1.6, 2.0, 3.4, 4.0, 5.4, 8.5. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70098, 70403, 70799, 70886, 70890. Corresponds to nt 473207-508488 of the sequence of chromosome VIII. |
| References |
Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151
Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229
Linda Riles, personal communication
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