Restriction digests of the clone give the following sizes (kb): BamHI--3.3; EcoRI--3.3; HindIII--3.3. Expression vector permitting visual detection of recombinants by lacZalpha complementation. The order of the major features in the plasmid is: ampR - pMB1 ori - 5' lacZ' - SacI/MCS/KpnI - 3' lacZ' - NdeI. |
