This is a plasmid vector containing a non-autonomous P element from Drosophila melanogaster. It should be used with a helper plasmid such as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237).
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Spradling AC, Rubin GM. Transposition of cloned P elements into Drosophila germ line chromosomes. Science 218: 341-347, 1982. PubMed: 6289435
Rubin GM, Spradling AC. Vectors for P element-mediated gene transfer in Drosophila. Nucleic Acids Res. 11: 6341-6351, 1983. PubMed: 6312420
