| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--18.0, 9.6, 7.8, 4.8, 2.0, 1.7, 1.4, 0.7; KpnI--32.0, 11.4, 6.7, 1.6; SalI--38.3, 8.4, 5.1; PstI--5.2, 5.1, 3.5, 3.5, 3.4, 3.2, 3.0, 2.8, 2.8, 2.3, 2.1, 2.0, 1.6, 1.5, 1.4, 1.4, 1.1, 0.9, 0.7, 0.7, 0.65, 0.65. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Cosmid contains coding sequences for both TNFalpha and TNFbeta; fragment containing both can be excised by KpnI/BamHI digest (approx. 7 kb). |
| References |
Spies T, et al. Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc. Natl. Acad. Sci. USA 83: 8699-8702, 1986. PubMed: 3464978
Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413
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