| Applications |
MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC. The antibody binds the VH1/V kappa 24 heavy and light chain idiotypic specificities of the phosphocholine specific myeloma protein, MOPC167. This antibody can be used as a typing reagent for mouse Ig bearing the VH1/V kappa 24 idiotypic specificity and can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of MOPC167 myeloma. |
| Comments |
Animals were immunized with MOPC167 and HPCM27 anti-phosphocholine (PC) antibodies. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody binds the VH1/V kappa 24 heavy and light chain idiotypic specificities of the phosphocholine specific myeloma protein, MOPC167. It completely inhibits the binding of MOPC167 to phosphocholine. It can bind the membrane receptor of 207-4 transgenic B cells and induce proliferation of these B cells. The antibody does not bind VH1/V kappa 22, VH1/V kappa 8, or VH1/V kappa 1 PC-binding proteins or other IgA or IgM myeloma proteins. This antibody can be used as a typing reagent for mouse Ig bearing the VH1/V kappa 24 idiotypic specificity and can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of MOPC167 myeloma. |
| References |
Sieckmann DG, et al. Anti-idiotype monoclonal antibodies specific for the MOPC167 anti-phosphocholine transgene-encoded antibody. Hybridoma 16: 503-511, 1997. PubMed: 9455702
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
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Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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