| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.75, 2.15; SalI--2.9, 2.05; PstI--4.3, 0.47, 0.32; PvuII--2.4, 1.05, 0.90, 0.64; HindIII--4.9. The insert contains the following restriction sites (approximate kb from the 5' end): SalI--0.16; SmaI--0.17; PvuII--0.74, 1.35; PstI--1.48, 1.92. Corresponds to nt 1-2141 of the published sequence, encoding the N-terminus of the protein. One of 4 clones (pI7-11, pF5-1, pA2-10, pA2-13, ATCC 65988-65995) that together contain the complete coding sequence. Cloned in the antisense direction. |
