| Insert |
DNA: cDNA Insert lengths(kb): 3.400000095367432 Tissue: colon carcinoma T84 cell line Gene product: cystic fibrosis transmembrane conductance regulator [CF] Alleles: A1, A1, A1, A1, A2, B1, B2, A1, A1, A2, B1, B2, A2, A2, A2, A2 |
| Comments |
This insert contains an internal EcoRI site. An EcoRI digest results in fragments of 2.1 and 1.3 kb from the insert. Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.1, 1.3; XbaI--6.4; HindIII--3.7, 0.87, 0.84; HindIII--3.7, 0.87, 0.84; PstI--6.4; EcoRI/XbaI--2.9, 2.1, 1.3. This clone includes exons 13 - 24 and hybridizes to genomic EcoRI fragments of 1.8, 1.8, 3.5, 1.9, 4.5, 2.4, 1.0, 2.8, 1.8, 8.5, 2.2, and 2.3 kb. |
| References |
Riordan JR, et al. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073, 1989. PubMed: 2475911
J R Riordan, personal communication
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