The insert contains the following restriction sites, in nt from the 5' end according to the EMBL record: EcoRI--19, BamHI--63; PstI--260; SacI--371; BglII--489. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.3, 0.02; BamHI--4.2, 0.1; SphI--4.3; PstI--4.0, 0.3; HindIII--4.3. Use of T3 RNA polymerase will yield the sense strand; T7 RNA polymerase will yield the complementary/antisense strand. |
