Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; EcoRI/HindIII--4.8, 2.4; HindIII--7.0. Promoter-cloning vector for S. cerevisiae using E. coli GusA activity as a reliable, sensitive reporter system that allows quantitative measurement of promoter activity. The 2 kb HindIII/EcoRI fragment described as the insert includes the 100 bp transcription terminator region generated by PCR. |
