Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.8; HindIII--3.7; XmnI--2.2, 1.4, 0.2; SmaI--3.0, 0.7; SphI--2.95, 0.75. The insert contains the following restriction sites (nt from the 5' end): SphI--43; SmaI--91, XmnI--653. Contains the complete coding sequence, and corresponds to nt 43-837 of the database record. The antisense strand is packaged. pEM303 (ATCC 79036/7) and pEM304 (ATCC 79038/9) differ only in the orientation of the insert. |
