-20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Introduction
Cell Line Description:
This cell line was initiated on September 13, 1994 from a patient who had received prior chemotherapy. The cell line took 4.5 months to establish. The tumor was classified as TNM stage IIA, grade 3, invasive ductal carcinoma.? Information regarding lymph node metastasis is not available.? The cells are poorly differentiated.? The cells are negative for expression of Her2-neu; they overexpress p53.? HCC1187 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of progesterone receptor (PR).? DNA isolated from an EBV-transformed lymphoblastoid cell line (HCC1187 BL) from the same patient is available as ATCC CRL-2323D.
Quality Control Specifications
> 100 kb in size by agarose gel electrophoresis
No RNA detected in the agarose gel
A260/A280 ratio ≥ 1.8
Tested and verified for PCR amplification and restriction digestion.
