Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.0; SmaI--5.7. The UMS sequences reduce background CAT transcription from the vector to levels approaching mock-transfected cells. A shuttle vector for assessing weak transcriptional activity of a cloned promoter using a CAT reporter gene. A 1023 bp SacI/XbaI fragment containing the poly(A) signal of mouse c-mos (UMS) was cloned into the NdeI site of pSV0cat. The NdeI site is 56 bp upstream of the SmaI cloning site. Constructed by J-P. Salier and K. Kurachi. |
