| 產(chǎn)品名稱 |
Avian reovirus type 3 |
| 商品貨號(hào) |
B224632 |
| Classification |
Reoviridae, Reovirus |
| Agent |
Avian reovirus type 3 |
| Strain |
59 |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Intestine from chicken with cloacal pasting |
| Product Format |
frozen |
| Storage Conditions |
?-70°C or colder |
| Comments |
Deposited as Avian reovirus type 3. Pathogenic for newborn mice. |
| Effect on Host |
CPE, syncytia formation ?CPE, syncytia formation |
| Recommended Host |
Primary chicken embryo fibroblasts Production Host: ?Primary CEF |
| Growth Conditions |
Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2 to 7 days in a humidified 5% CO2 atmosphere |
| Name of Depositor |
BL Patel, University of Minnesota, College of Veterinary Medicine |
| Source |
Intestine from chicken with cloacal pasting |
| References |
Deshmukh DR, Pomeroy BS. Avian reoviruses. I. Isolation and serological characterization. Avian Dis. 13: 239-243, 1969. PubMed: 4307737
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