| 產品名稱 |
45.1 DRbeta 008 |
| 商品貨號 |
B224679 |
| Designations |
45.1 DRbeta 008 |
| GenBank Number |
X03069
|
| Species |
Homo sapiens, human |
| Depositors |
EO Long |
| Vector |
Construct size (kb): 4.099999904632568 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.322999954223633 Tissue: hemizygous 45.1 cell line (DR1, DQw1, DPw2) Gene product: major histocompatibility complex, class II, DR beta 1(HLA class II histocompatibility antigen, DR-1 beta chain) [HLA-DR1B] |
| Insert Size (kb) |
1.323 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
BamHI site at 3' end is within SV40 sequence (coordinates 0.715-0.710). Insert cut out with BamHI will include 35 bp of SV40. Restriction digests of the clone give the following sizes (kb): BamHI--2.7, 1.4; HindIII--2.7, 1.4; SalI--3.7, 0.46. Insert contains 62 bp 5' untranslated sequence. The first nucleotide of the insert is 26 bp downstream from TATA. Contains the complete coding sequence. |
| References |
Klohe EP, et al. Analysis of the molecular specificities of anti-class II monoclonal antibodies by using L cell transfectants expressing HLA class II molecules. J. Immunol. 141: 2158-2164, 1988. PubMed: 2459201
Tonnelle C, et al. DObeta: a new beta chain gene in HLA-D with a distinct regulation of expression. EMBO J. 4: 2839-2847, 1985. PubMed: 2998758
|
