| 產品名稱 |
alpha13 pcDNAI |
| 商品貨號 |
B224777 |
| Designations |
alpha13 pcDNAI |
| Species |
Mus musculus, mouse |
| Depositors |
HR Bourne |
| Applications |
contains sequence G protein, alpha subunit |
| Vector |
Construct size (kb): 6.5 |
| Insert |
DNA: cDNA Insert lengths(kb): 2.5 Gene product: G protein, alpha subunit Target Gene: G protein, alpha subunit |
| Insert Size (kb) |
2.5 |
| Media |
ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.0, 0.87, 0.6; HindIII/XbaI--4.0, 2.0, 0.46; Xho--6.5. Expresses protein in mammalian cells. XhoI site in vector polylinker was lost during cloning, but there are two internal XhoI sites in insert. T7 promoter is the closest to 5' end. Alpha13 excised from pMOB and subcloned into pcDNAI using EcoRI and NsiI sites. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Voyno-Yasenetskaya T, et al. G alpha 13 stimulates Na-H exchange. J. Biol. Chem. 269: 4721-4724, 1994. PubMed: 8106440
|
