Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3, 1.8, 0.5; EcoRV--4.2, 1.5; HindIII--4.5, 1.3. Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
