| Insert |
DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy [D14S24] Alleles: B1, B2, C1, C2, A1, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--17.0, 7.4, 6.4, 5.0, 3.2, 2.9, 2.7, 2.5, 1.6, 1.4, 1.2; BamHI--uncut; PstI--12.0, 6.2, 6.2, 3.6, 3.0, 2.7, 1.8, 1.8, 1.6, 1.4 + smaller; EcoRI--17.0, 7.0, 5.5, 5.0, 1.7, 1.6, 1.4, 1.3, 1.1, 0.9. The insert can be excised from the vector with EcoRI, resulting in a 5.5 kb EcoRI vector fragment. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The MspI polymorphisms have a combined PIC value of 0.71 and a combined heterozygosity of 76%. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, HincII, PstI, RsaI, TaqI (panel of 5 individuals). |
| References |
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
Bowden DW, et al. Identification and characterization of 23 RFLP loci by screening random cosmid genomic clones. Am. J. Hum. Genet. 44: 671-678, 1989. PubMed: 2565079
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