Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 0.6; HindIII--3.6, 0.2; EcoRI--3.0, 0.8; XbaI--3.8; PstI--3.4, 0.4. The insert contains the following restriction sites (nt position according to the published sequence): NcoI--1356; PstI--1613; BamHI--1759; HindIII--1833. Detects human genomic EcoRI fragments of 2.3, 1.8, and 1.3 kb. Contains the 3' end of the cDNA, beginning at the EcoRI site at nt 1223 of the published sequence. Overlaps P5 (ATCC 67560, 65761) by 507 bp. Oriented with the 5' end adjacent to the T3 promoter. |
