Restriction digests of the clone give the following sizes (kb): HindIII/KpnI--3.3, 1.94, 1.25; HindIII--3.45, 3.2; BamHI--4.0, 2.8; KpnI--4.36, 2.03; consistent with deposited DNA. The fragment is within the DMD locus. A HindIII/KpnI digestion yields 2 fragments--one at 1.8-1.9 and the other at 1.15. The latter detects the RFLP, the former is slightly repeated. The polymorphisms detected by this probe are contained within the DMD locus. Care should be taken to assure efficient transfer of the larger allele. |
